Asparagine quantification in cellular culture media using copper modified carbon nanotubes composite electrodes

LOPEZ RODRIGUEZ, MARÍA LORENA; LUQUE, GUILLERMINA; FELICE, CARMELO JOSÉ; FERREYRA, NANCY F.; MADRID, ROSSANA ELENA; RIVAS, GUSTAVO A.; GIACOMELLI, CLAUDIA E.

SENSORS AND ACTUATORS B-CHEMICAL

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2011 vol. 158 p. 423 - 426

0925-4005

Direct quantification of Asparagine (Asp) in a Streptomyces sp. M7 (SM7) culture medium was performed using multi-wall carbon nanotubes composite electrodes modified with copper microparticles (CNTPECu). A sensitive and fast response is achieved at CNTPE-Cu after additions of SM7 cultures supernatant, without interference of the culture medium components. This response allows determining the Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacteria consume Asp as a carbon and nitrogen sources in the absence of another carbon source such as glucos.