POROUS SILICA NANOPARTICLES LOADED WITH NORFLOXACIN

T. RODRIGUEZ ÁLVAREZ; J. ORTIZ OTALVARO; M. AVENA; V. LASSALLE; M.S. VELA GUROVIC; M. BRIGANTE; M. AGOTEGARAY

Buenos Aires

Congreso; XI REUNIÓN ANUAL DE LA ASOCIACIÓN ARGENTINA DE NANOMEDICINAS (NANOMED-AR); 2021

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

The effect of raw magnetic mesoporous silica nanoparticles (MMSiO2) and 4.97% Norfloxacin-loaded MMSiO2 (NFX-MMSiO2) was tested on an E.coli bacterial growth to evaluate inhibitory activity. MMSiO2, with a core (Fe3O4)-shell type structure, were prepared by a modification of the Stöber method. The average size, surface area and isoelectric point was 125 nm, 471 m2g-1 and 2.4, respectively. A suitable range of concentrations of NFX-MMSiO2 [16 μg/mL, 8 μg/mL, 4 μg/mL, 2 μg/mL] was seeking to the minimum inhibitory concentrations known by NFX. Microdilution inhibitory assay was conducted for of MMSiO2, NFX-MMSiO2 and NFX. The samples were incubated for 18h at physiologic temperature of 37°C and absorbances were measured in a Multiskan GO VWR Co Microplate Spectrophotometer, Thermo Scientific. The results were standardized subtracting the absorbances of the vehicle and data were statistically analyzed.The evidence suggested differences in the inhibitory activities depending on NFX-MMSiO2 concentrations when compared to NFX. At low doses, the major prevalence of growth decrease was observed for NFX-MMSiO2 in comparison to the same doses of the antibiotic, whereas at higher concentrations of NFX-MMSiO2 the effect decreased in comparison to free NFX. There was exposed a decline in the inhibitory growth activity of NFX according to its minimum inhibitory concentration (MIC) in contrast to MMSiO2 which activity remained constant. It was also observed that MMSiO2 did not present a direct effect on the bacteria. However, these NPs could have enhanced the antibiotic effect at lower doses. This fact may be due to the outcome of iron from the NPs and to changes in the ionic force of growing medium. Results demonstrate that loading NFX into MMSiO2 would be a strategy to target the drug to specific sites in the organism under the influence of an external magnetic field, at low concentration to ensure a better performance in comparison to free NFX.Observaciones: Este congreso es de alcance internacional y nuclea a científicos de diferentes especialidades y países, por lo que es un evento afín a la especialidad o a linea de investigación llevada a cabo. La participación del Dr. Brigante en el evento es protagónica ya que se encuentra como supervisor de la linea de investigación llevada acabo en conjunto con el grupo de la Dra. Agotegaray.