PROTEIN COATING DEFINES THE BIOLOGICAL IDENTITY OF NANOPARTICLES: RELEVANCE OF PROTEIN- PROTEIN INTERACTIONS.

CECILIA VASTI; LAURA BONNET; MAURICIO R. GALIANO; RICARDO ROJAS; CARLA E. GIACOMELLI

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

In the area of biomedicine, the use of nanoparticles(NPs) as carriers of therapeutic or theranostic agents has increasedin the last years. Therefore, it is mandatory to understand the interactionbetween NPs and living organisms. In contact with biologicalfluids, the surface of NPs (synthetic identity) is covered with biomolecules(mainly proteins) that form a protein corona, which definesthe biological identity. The protein corona formation is mediated bynon-specific physical interactions, common to all molecular systems(van der Waals, electrostatics, etc.) as well as protein-protein interactions(PPI). This work is aimed at studying the protein coronaformation on layered double hydroxides nanoparticles (LDH-NPs)previously modified with either albumin (LDH@ALB) or fetal bovineserum (LDH@FBS) and the cellular response of the biologicalidentity. With such a purpose, LDH@ALB and LDH@FBS were dispersedin a protein containing cell culture medium, to compare theinterfacial properties of the synthetic and biological identities andto characterize the protein corona. The interfacial properties weredetermined by dynamic light scattering measuring the size distributionand zeta potential. The molecules of the protein corona wereidentified by liquid chromatography coupled to mass spectrometryand the PPI network was evaluated with a novel approach, basedon the bioinformatic tool STRING v.10.0 (http: //www.string-db.org/).The cellular response was evaluated through cytotoxicity and internalizationexperiments. We demonstrate that the protein corona formationwas guided by PPI, through bioaffinity recognition, which ledto different networks depending on the surface properties, definedby the protein coating. Furthermore, LDH@ALB and LDH@FBS arenot cytotoxic and both present high levels of cellular internalization.Keyword: protein corona, biological identity, PPI network.