INVESTIGADORES
BERLI Claudio Luis Alberto
congresos y reuniones científicas
Título:
DNA electrophoresis through micropores manufactures by laser ablation
Autor/es:
M.S. PEREZ, B. LERNER, J.A. LELL, A. FASCISZ ZEBALLOS, B. CETRALONGO, C. LASORSA, C.A. RINALDI, A. BOSELLI, A. LAMAGNA; P.A. KLER, C.L.A. BERLI
Lugar:
Nürnberg/Nuremberg
Reunión:
Congreso; IMCS 2012 - The 14th International Meeting on Chemical Sensors; 2012
Institución organizadora:
University of Bayreuth
Resumen:
The work presents an alternative method for the manufacture of nano/micro/pores by using the combination of laser ablation and wet etching. The process of laser ablation was done on silicon (Si) wafers with silicon nitride (Si3N4) used as a sacrificial layer. The size of the pores was carefully controlled by following an optical technique. The method exhibits a series of advantages in relation micromachining techniques previously used. The fabricated pores were then integrated to PDMS microchannels, which constitutes a novel setup to be considered for microfluidic applications. Furthermore, as the system is addressed to study the electrically-driven transport of macromolecules, DNA electrophoresis was performed in the fabricated microsystems to prove the principle. Also for these purposes, the electric field throughout the microchannel and pore region was studied in details by using numerical simulations.