FERNANDINO Juan Ignacio
congresos y reuniones científicas
Spatiotemporal expression pattern of the medaka fish (Oryzias latipes) Ndrg family during embryonic development stages
ARIAS PADILLA, L.F.; FERNANDINO, J.I.
Congreso; IX LATIN AMERICAN SOCIETY FOR DEVELOPMENTAL BIOLOGY MEETING 2017; 2017
LATIN AMERICAN SOCIETY FOR DEVELOPMENTAL BIOLOGY
The N-myc downstream regulated gene (Ndrg) family consists of four main members Ndrg1, 2, 3, and 4. Which are well conserved through evolution. The function and regulation of the Ndrg family is beginning to emerge, and are involved in many vital biological events including development. However, the exact function of this family during vertebrate embryogenesis especially in non-mammalian vertebrate remains largely unknown. Here, we described the characterization and spatiotemporal expression pattern by Whole Mount in situ Hybridization (WMISH) of Medaka fish Ndrg gene family, including ndrg1a, ndrg1b, ndrg2, ndrg3a, ndrg3b, during embryonic developmental stages. The expression of ndrg1a started first in 2-somite stage (st. 19) until hatching stage (st. 39), mainly in otic vesicle, pronephros, nephric conduct, gastrointestinal tract and brain. The expression of ndrg1b started first in somite completion stage (st. 32) until hatching stage (st. 39) principally in gonads. The expression of ndrg2 started first in 22 somite stage (st. 26) until st. 39, principally in nephric conduct, brain and pectoral fins. ndrg3a expression started first in 9-somite stage (st. 22) until hatching stage (st. 39), mostly in nephric conduct, brain and notochord. Finally expression of ndrg3b started first in st. 26 until st. 39, principally in brain and gonads. Overall, these differential expression patterns of medaka fish ndrg genes suggest that perhaps play distinct role throughout the embryonic development by controlling organ-specific development. Our study provides new evidence that ndrg family is involved in development in non-mammalian vertebrate.