Localization of pituitary and extrapituitary gonadotropins by in situ hibridization in pejerrey Odontesthes bonariensis

ELISIO, M.; JUAN IGNACIO FERNANDINO; GUSTAVO MANUEL SOMOZA; LEANDRO ANDRES MIRANDA

Buenos Aires

Jornada; XI Jornadas de la Sociedad Argentina de Biología; 2009

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0in; margin-right:0in; margin-bottom:10.0pt; margin-left:0in; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-fareast-font-family:Calibri; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:ES-TRAD;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ascii-font-family:Calibri; mso-fareast-font-family:Calibri; mso-hansi-font-family:Calibri;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> In a previous study by RT-PCR it was demonstrated the presence of α and β gonadotropins (GtHs) subunits in pejerrey brain and gonads. In this work, using in situ hybridization, it was possible to identify these transcripts in the brain, pituitary, and gonads of adult pejerrey of both sexes. As it was expected, the three GtHs subunits RNAm were detected in cells of pituitary pars distalis proximalis and pars intermedia and conspicuously in neurons of nucleous lateralis lemniscus. The three GtHs subunits were identified in testicular spermatogonia and spermatocytes, whereas in the ovary they were observed in oocytes at different developmental stages. In spite of, extrapituitary GtHs function are not known yet, these results suggest that they can play novel roles acting as brain neuromodulator and as regulators of the gonads.