FERNANDINO Juan Ignacio
congresos y reuniones científicas
N-myc downstream regulated 3 (ndrg3): characterization during primorial germ cell proliferation and regulation by androgen in medaka
ARIAS PADILLA, L.F.; FERNANDINO, J.I.
Simposio; 10th International Symposium on the Reproductive Physiology of Fish; 2014
Introduction In medaka primordial germ cells (PGCs) develop through various stages of mihotic proliferation, a process highly regulated in a sexually dimorphic manner. In females, the number of PGCs is increased dramatically after stage 37, previous to hatching, meanwhile in males the number of PGCs can be observed from the second weed after hatching. The desregulation of the PGCs proliferation can produce a male-to-female sex reversal, e.g. hotei mutant. Methods To evaluate if ndrg3 could be involved on the gonadogenesis was analyzed its expression during different stages of gonadal development by RT-qPCR using medaka (Oryzias latipes, himedaka stain). Furthermore, the regulation of ndrg3 expression by androgen (11-ketotestosterone, 11-KT) was analyzed using an ex-vivo approach. Results and Discussion A pattern of differential expression between sexes, with significant differences in stage 37 in females and 14 and 30 days post hatching in male was obtained. For both sexes, the increased of ndrg3 expression was correlated with the time of proliferation PGCs characterized for this species. Moreover, the expression of ndrg3 was up-regulate with 11-KT exposition. Conclusion The ndrg3 expression at different stages of medaka showed significant temporary differences between sexes, with an increase in expression upon proliferation of PGCs of both sexes, so that might be involved in this process. In turn, ndrg3 expression was positively regulated by androgen, suggesting a regulation by sex steroids.