INVESTIGADORES
PITTA-ALVAREZ Sandra Irene
congresos y reuniones científicas
Título:
Regeneration protocol from transformed roots of Petunia axillaris (Lam.) Britton, Stern & Poggenb., first assays.
Autor/es:
MARIANA PÉREZ DE LA TORRE; VERÓNICA BELLA ; ADRIANA KATO; NATALIA AYUB; SANDRA I. PITTA-ALVAREZ
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Annual Meeting of the Argentine Society for Biochemical and Molecular Biology Research; 2012
Institución organizadora:
SAIB
Resumen:
In the Floriculture Institute, within a native Solanaceae breeding program, we started to work with transformation techniques to obtain more compact Petunia plants. In the international market, potted plants have more acceptation and commercial value; therefore the development of compact national varieties is relevant for local producers. In this project we used one genotype of P. axillaris, maintained in vitro. The transformation was made with Agrobacterium rhizogenes LBA15834 over leaf laminae and petioles in MS/2 medium supplemented with 30 g/l sucrose and 7.5 g/l agar. Transformation was confirmed so far, in the hairy roots obtained, by PCR reaction with rol A, rol B, rol C, rol D and vir C primers. For the regeneration protocol we used nine different combinations of ANA and BAP hormones. The combinations were: MS1: Control, MS2: 0.5 mg/l BAP, MS3: 2 mg/l BAP, MS4: 0.5 mg/l ANA, MS5: 2 mg/l ANA, MS6: 0.5 mg/L ANA + 0.5 mg/L BAP, MS7: 0.5 mg/l ANA + 2 mg/l BAP, MS8: 2 mg/l ANA + 0.5 mg/l BAP and MS9: 2 mg/L ANA + 2 mg/l BAP. The preliminary results showed the MS4, MS6 and MS8 as most promising combinations. The obtained calluses of these combinations were transferred to MS PGR-free medium containing 30 g/l sucrose and 7.5 g/l agar, to begin with the plantlets regeneration. At moment we are in this last regeneration step.