INVESTIGADORES
PITTA-ALVAREZ Sandra Irene
congresos y reuniones científicas
Título:
Breeding program in native Argentine Solanaceae: towards more compact plants through trangenesis techniques
Autor/es:
PEREZ DE LA TORRE, M.; ALDERETE, M.; MATA, D.; HAGIWARA, J.C.; MATA, D.; SCOLARI, F. Y PITTA-ALVAREZ, S.I.
Lugar:
CABA
Reunión:
Simposio; VII International Symposium on New Floricultural Crops; 2011
Institución organizadora:
Instituto de Floricultura, INTA
Resumen:
In the ornamental plant market, potted plants that have a compact appearance are preferred by the general public. Many Argentine native species with ornamental potential tend to exhibit elongated growth habits. This effect is usually reverted using chemical substances that are known to be damaging to the environment and to health. In this research, a different approach, based on transgenic techniques, was tested. The ornamental plant used as a model was Petunia, in particular two native genotypes: P. integrifolia and P. axillaris. Both genotypes were first multiplied by micropropagation techniques. Once sufficient biomass was obtained, two different strategies were employed: 1) transformation with Agrobacterium rhizogenes and 2) transformation with an A. tumefaciens containing the plasmid pCHF3.35s::CBF1. In the first case, it has been widely observed that plants regenerated from transformed roots obtained by infection with A. rhizogenes exhibit a dwarf-like appearance, achieving thus the compact qualities desired. In the second strategy, the cbf1 gene inhibits plant growth, probably due to interference in the gibberellin synthesis route. Both approaches will hypothetically lead us to obtain the plants that are required by the market. This presentation will describe and discuss the advances made in this research and the potential of both strategies.