Micropropagation protocol for coastal quinoa

REGALADO, J. J.; TOSSI, V. E.; BURRIEZA, H. P.; ENCINA, CL; PITTA-ALVAREZ, S. I.

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Lugar: Berlin; Año: 2020

0167-6857

Quinoa is a model halophyte plant, with seeds rich in proteins, fatty acids and lacking prolamines. There are two types ofquinoa cultivars: highland and coastal. Coastal cultivars are used in breeding programs for warm-season quinoa production.Diferent biotechnological tools are useful for breeding and understanding the mechanisms involved in abiotic stress tolerance. However, micropropagation protocols have been developed only for highland cultivars. The aim of this research wasto develop a micropropagation protocol for coastal cultivars. The initial explants were apical segments from two coastalcultivars (Cahuil and Villa). The regeneration rate in regeneration quinoa media 3 (QM-3: MS supplemented with 1 mg l−1KIN and 1 mg l−1 BA) was acceptable (93±5% for Cahuil and 79±8% for Villa), but the number of regenerated shoots waslow (2.14±0.93 and 1.43±0.73 respectively). We included a shoot multiplication stage using regeneration quinoa medium1 (QM-1: MS supplemented with 2 mg l−1 BA), obtaining 7.96±2.92 shoots per explant for Cahuil and 4.10±3.00 for Villa.Also, we obtained a higher number of acclimatized quinoa using ex vitro shoot rooting (73±8% for Cahuil and 77±8% forVilla) than in vitro shoot rooting (in quinoa rooting media 3 RQM-3, MS supplemented with 2 mg l−1 IBA: 87±6% for Cahuiland 83±7% for Villa) and subsequent acclimatization (83±7% and 80±7% respectively). Furthermore, ex vitro rootingcombines rooting and acclimatization in one step and requires less time. This is the frst coastal cultivar micropropagationprotocol reported and the starting point for development of other biotechnological tools