Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline

PITTA-ALVAREZ, S.I.; SPOLLANSKY, T.C.; GIUILIETTI, ANA MARÍA

BIOTECHNOLOGY LETTERS

SPRINGER

Lugar: Berlin; Año: 2000 vol. 22 p. 1653 - 1656

0141-5492

CaCl2 (50 mM) and hemicellulase (0.5 U mg􀀀1) increased the intracellular accumulation (60?250%), release(60?200%) and production (45?200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida.Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in increasing hyoscyamineand scopolamine production.