INVESTIGADORES
PITTA-ALVAREZ Sandra Irene
artículos
Título:
Biotransformation of Hydroquinone by. Hairy Roots of Brugmansiac andida and Effec: t of Sugars and Free-Radical Scavengers
Autor/es:
CASAS, DANIELA ANDREA; PITTA ALVAREZ, SANDRA; GIUILIETTI, ANA MARÍA
Revista:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Editorial:
HUMANA PRESS INC
Referencias:
Lugar: Oregon; Año: 1998 vol. 69 p. 127 - 136
ISSN:
0273-2289
Resumen:
Hairy roots of Brugmansia candida were used to bioconvert hydroquinoneinto arbutin. The highest bioconversion, with the lowest damageto the cells, was attained when concentrations of 20-40 mg/Lhydroquinone were used. Sugars (sucrose, glucose, mannitol, and sorbitol)at concentrations of 30-120 g/L enhanced bioconversion, and, ofthese, sucrose was the most effective. Two different free-radical scavengerswere also tested: sodium benzoate and gallic acid. The first onediminished biotransformation efficiency; gallic acid did not affect biotransformationat all. Preliminary permeabilization treatments testedfailed to liberate arbutin into the medium, and provoked a total loss incell viability.
