Hairy Roots of Brugmansia candida That Grow without Agitation: Biotechnological Implications

PITTA ALVAREZ, SANDRA; GIUILIETTI, ANA MARÍA

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Lugar: Washington; Año: 2001 vol. 17 p. 661 - 663

8756-7938

Hairy roots of Brugmansia candida that grew without agitation were obtained. Kineticsof growth and production of the tropane alkaloids scopolamine and hyoscyamine, withand without agitation, were studied. The exponential growth rate was higher in theroots that were exposed to shaking (0.13 d-1) than in the nonagitated ones (0.09 d-1).The specific production and the levels per flask of both alkaloids were enhanced withoutshaking. The use of these roots in large-scale productions could be economicallyadvantageous. It remains to be seen if the data obtained in shake flasks can beextrapolated to large-scale bioreactors.