EFFECTS OF GIBBERELLIN GA 7 ON KINETICS OF GROWTH AND TROPANE ALKALOID ACCUMULATION IN HAIRY ROOTS OF BRUGMANSIA CANDIDA

SI PITTA-ALVAREZ; GIUILIETTI, ANA MARÍA

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT

SPRINGER

Lugar: Berlin; Año: 1997 vol. 33 p. 147 - 153

1054-5476

Brugmansia candida, an indigenous South American plant, produces the tropane alkaloids scopolamine and hyoscyamine,which are widely employed in medicine as anticholinergic agents. In this research, hairy roots ofBrugmansia candida,obtained through infection with Agrobacterium rhizogenes LBA 9402, were employed to produce these tropane alkaloids invitro. The effects of different concentrations of GA: on kinetics of growth and alkaloid accumulation on two different hairyroot clones of B. candida were analyzed, and the influence of GA: on the number of new branches and rates of elongationwas also studied. On clone 7A, GA7 at concentrations of 10 -~, 10 -1, and I rag/1 increased the exponential growth rate.Levels of 10-~ and 10 ~ mg/1 GA 7 elevated the scopolamine/hyoscyamine (S/H) ratios in the early phases of growth, butthe sum of scopolamine plus hyoscyamine per flask (S + H) decreased during that period. When 1 rag/1 GA: was used, thehighest S/H ratios were observed in late exponentiagearly stationary phases, but the highest S + H totals were obtained inmid-exponential phase. GA~ at levels of 10 -1 and, especially, 1 rag/1 exerted a positive effect on formation, emergence,and rate of elongation of lateral roots (clone 7A). On clone 7B, levels of 10 ~ and 1 rag/1 GA: did not alter significantlythe exponential growth rate. GA7 in concentrations of 10-~ mg/l induced increases in both S/H ratio and S + H totals inlate phases of growth.