INVESTIGADORES
LIBKIND FRATI Diego
congresos y reuniones científicas
Título:
Carotenoid pigments enhance photoprotection in the yeast Rhodotorula mucilaginosa
Autor/es:
MOLINÉ, M; LIBKIND FRATI, D; DIÉGUEZ, MC; VAN BROOCK, MR
Lugar:
Miramar, Arg
Reunión:
Congreso; Reunión SAIB; 2005
Resumen:
We analyze the relationship
between carotenoid pigments and UV-B resistance in strains of the yeast Rh. mucilaginosa.Carotenoid and phytoene (a
carotenoid precursor) production as well as the cell survival to UV-B exposure
were studied using a set of 13 strains both type strain and Patagonian native
isolates. Albino and hyper pigmented mutants of one selected strain were
obtained after UV-B mutagenesis and characterized by molecular
(PCR-fingerprinting) and conventional techniques. These strains were studied as
described above and compared with T type. Data from the first screening were submitted
to principal component analysis showing a strong relationship between total
carotenoids concentration and UV-B survival, while phytoene concentration did
not co-relate with these two variables (carotenoids and resistance). In the
second assay the albino mutant presented similar UV-B survivorship than the
parental strain while the hyper pigmented (300% increase in total carotenoid)
showed a consistent upgraded survival (250%). These results support the idea that enhanced
carotenoid concentration in pigmented yeasts could be responsible for
survivorship and play a role as UV-B resistance mechanism.