Characterisation of the N gene of a groundnut ringspot virus (GRSV) isolate from Argentina.

R. A. DEWEY; L SEMORILE; O GRAU; AC DE AVILA; EW KITAJIMA

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT

Wiley

Año: 1995 vol. 143 p. 713 - 717

0931-1785

This paper describes the characterization of a Tospotirus isolate (M316) from Mendoza, Argentina. The N gene was cloned and sequenced. The sequence obtained was compared with the N gene sequences of other tospoviruses. The percentages of the nucleotide homology and amino acid similarity showed that this isolate may be considered a member of the species groundnut ringspot virus (GRSV). The conclusion drawn from the molecular studies was confirmed by DAS-ELISA and western blotting. This is thefirst report of GRSV in Argentina, previously described for a South African (SA-05) and Brazilian (BR-08) isolates.