ROLDAN OLARTE Eugenia Mariela
congresos y reuniones científicas
Detection of urokinase type plasminogen activator receptor (u-PAR) in oocyte and porcine oviduct
ROLDÁN OLARTE, M; JIMÉNEZ DÍAZ, M; GARCÍA, D; MICELI, D.C
Tafi del Valle, Tucumán
Jornada; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2007
Asociación de Biología de Tucumán
Plasminogen activators (PAs) are highly specific proteolytic enzymes that convert plasminogen (PIg) to plasmin (PI). Two types of PAs are known: tissue type (t-PA) and urokinase type (u-PA). The u-PA receptor (u-PAR) is present in the plasma membrane of several cell types, the u-PA/u-PAR complex permits the focalized PI generation in a few areas of cellular surfaces and can active intracellular signal transduction system. We demonstrated that porcine oviduct synthesizes PAs, in this work we analyzed the u-PAR presence in oocytes and porcine oviducts. Oocytes, obtained by aspiration of ovaries from recently slaughter housed sows, were selected, fixed and then analyzed by indirect immuno-fluorescence. An intense fluorescence over oocyte surface, but not in zona pellucida was observed. Considering that PIg was previously detected in porcine oocytes, these results suggest that u-PA actives PIg near the oocyte surface. By indirect immunohisto-chemistry we also analized porcine oviducts, detecting u-PAR in epithelial and muscular cells but not in stroma cells from ampulla and isthmus. The presence of u-PAR in epithelial cells indicates that this molecule could bind the u-PA present in the oviductal lumen; the generation of PI on the epithelial surface and the activation of intracellular signals would be important to the fertilization or early embryo development.