“Identification of molecular genetic markers by means of RAPD in Vicuña genome”

LONGO, A.E; ROLDÁN-OLARTE, M; VALDECANTOS, P.A; GARCÍA, D.C; MICELI, D. C

Tafi del Valle, Tucumán

Jornada; XXIV Jornadas Científicas de la Asociación de Biología de Tucumán; 2008

Asociación de Biología de Tucumán

The vicu (Vicugna vicugna) is a wild South American Camelid. She was on the verge of extinction because of was hunted for his fine fibre. Nowadays is not know the genetic variability of vicuña populations of Argentina. In this work we used RAPD molecular markers in order to study the genetic variability of INTA Abra Pampa (Jujuy) vicuña population and to identify news markers. The Random Amplified Polymorphic DNA (RAPD) technique uses only primer of 10 nucleotides and his hybridization is to low temperature. Two strategies were use for generating polymorphic patterns: 1) RAPD with primers of 10 nucleotides and arbitrary sequence (Series A, Biodynamics), 2) RAPD whit primers of 20 nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI, TGF βII, BMP 15 and uPAR). The PCR reactions were realized by genomic DNA obtained from blood and fibers to a temperature of hibridization of 35ｰC. The amplifications products by possible polymorphism were selected and isolated of the gel for his later purification, cloning and sequence. The selected bands are moleculars markers potentials and they can help to know more about the genetic variability of vicu populations and her conservation. Camelid. She was on the verge of extinction because of was hunted for his fine fibre. Nowadays is not know the genetic variability of vicuña populations of Argentina. In this work we used RAPD molecular markers in order to study the genetic variability of INTA Abra Pampa (Jujuy) vicuña population and to identify news markers. The Random Amplified Polymorphic DNA (RAPD) technique uses only primer of 10 nucleotides and his hybridization is to low temperature. Two strategies were use for generating polymorphic patterns: 1) RAPD with primers of 10 nucleotides and arbitrary sequence (Series A, Biodynamics), 2) RAPD whit primers of 20 nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI, TGF βII, BMP 15 and uPAR). The PCR reactions were realized by genomic DNA obtained from blood and fibers to a temperature of hibridization of 35ｰC. The amplifications products by possible polymorphism were selected and isolated of the gel for his later purification, cloning and sequence. The selected bands are moleculars markers potentials and they can help to know more about the genetic variability of vicu populations and her conservation. Camelid. She was on the verge of extinction because of was hunted for his fine fibre. Nowadays is not know the genetic variability of vicuña populations of Argentina. In this work we used RAPD molecular markers in order to study the genetic variability of INTA Abra Pampa (Jujuy) vicuña population and to identify news markers. The Random Amplified Polymorphic DNA (RAPD) technique uses only primer of 10 nucleotides and his hybridization is to low temperature. Two strategies were use for generating polymorphic patterns: 1) RAPD with primers of 10 nucleotides and arbitrary sequence (Series A, Biodynamics), 2) RAPD whit primers of 20 nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI, TGF βII, BMP 15 and uPAR). The PCR reactions were realized by genomic DNA obtained from blood and fibers to a temperature of hibridization of 35ｰC. The amplifications products by possible polymorphism were selected and isolated of the gel for his later purification, cloning and sequence. The selected bands are moleculars markers potentials and they can help to know more about the genetic variability of vicu populations and her conservation. Vicugna vicugna) is a wild South American Camelid. She was on the verge of extinction because of was hunted for his fine fibre. Nowadays is not know the genetic variability of vicuña populations of Argentina. In this work we used RAPD molecular markers in order to study the genetic variability of INTA Abra Pampa (Jujuy) vicuña population and to identify news markers. The Random Amplified Polymorphic DNA (RAPD) technique uses only primer of 10 nucleotides and his hybridization is to low temperature. Two strategies were use for generating polymorphic patterns: 1) RAPD with primers of 10 nucleotides and arbitrary sequence (Series A, Biodynamics), 2) RAPD whit primers of 20 nucleotides whit 60-70% of C+G in the 3´ extreme (TGF βI, TGF βII, BMP 15 and uPAR). The PCR reactions were realized by genomic DNA obtained from blood and fibers to a temperature of hibridization of 35ｰC. The amplifications products by possible polymorphism were selected and isolated of the gel for his later purification, cloning and sequence. The selected bands are moleculars markers potentials and they can help to know more about the genetic variability of vicu populations and her conservation.