INVESTIGADORES
PESCARETTI Maria De Las Mercedes
congresos y reuniones científicas
Título:
THE MODULATION OF THE Salmonella bapA GENE EXPRESSION BY RCSCDB SYSTEM ACTIVATION DEPENDS ON GROWTH MEDIA AND CONTACT-SURFACE
Autor/es:
INDIANA M PÁEZ PAZ; MARIA CECILIA LECCESE TERRAF; MARÍA DE LAS MERCEDES PESCARETTI; MÓNICA A. DELGADO
Lugar:
Tafi del Valle, Tucuman
Reunión:
Congreso; XXXV Jornadas Científicas Asociacion de Biologia de Tucuman; 2018
Resumen:
Biofilms are described as polymeric matrix of extracellular substances in which the microorganisms are embedded, and attached to a living or artificial surface. It has been reported that the RcsCDB regulatory system participates in biofilm development. This system responds to cell surface stresses affecting the membrane integrity and producing the system activation. Here, we studied the regulation of the Salmonella Typhimurium biofilm formation under different growth media and surfaces conditions. For this purpose, we used the transcriptional lacZY fusion expression to chromosomal bapA gene, an RcsB-dependent gene required for this bacterial lifestyle. The ß-galactosidase activity was quantified under LB, LB without salt (LBsS), LBsS supplemented with biliar salts, planktonic or biofilm culture conditions. In addition, we test the bapA expression by ß-galactosidase activity from biofilm developed on glass and polystyrene surfaces. We observed that the dissimilar expression levels of bapA on different media, growth and contact-surface conditions are controlled by RcsCDB system. These findings contribute to the understanding of the biofilm development and the important role of the RcsCDB system in this bacterial phenotype.