CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
Enhancement of anthraquinone production in Morinda citrifolia cell suspension cultures after treatment with azetidine-2-carboxylic acid and thiazolidine-4-carboxylic acid
Autor/es:
QUEVEDO, C.; PERASSOLO, M; GIULIETTI A M; RODRIGUEZ TALOU, J
Lugar:
Barcelona
Reunión:
Congreso; 14th European Congress on Biotechnology,; 2009
Institución organizadora:
European Federation of Biotechnology
Resumen:
Plant cell suspension cultures are attractive alternatives for large
scale production of plant-derived natural products, in particular of
secondary metabolites. Morinda citrifolia is a member of Rubiaceae
family that produces anthraquinones (AQs), anthracene derivatives
which exhibit biological interesting properties. The basal
compound, anthraquinone (9,10-dioxoanthracene), can be substituted
in various ways, resulting in a great diversity of structures.
Different metabolic routes are involved in AQs synthesis: shikimate
pathway produces chorismic acid, which is then converted
into isochorismic acid by the enzyme isochorismate synthase. The
reaction of this compound with _-ketoglutaric acid generates osuccinylbenzoic
acid, the precursor of A and B rings of the AQs
structure. C ring is derived from an isoprene unit produced by the
2-C-methyl-D-erythritol-4-phosphate pathway. The proline cycle
was proposed to be linked to the pentose phosphate pathway
(PPP), as the first one generates two NADP+ which are cofactors
of the two first enzymes in the PPP. The PPP produces erithrose-4-
phosphate, which is substrate of the shikimate pathway. The aim
of this work was to study a possible link between proline cycle
and AQs production and to evaluate this link as a possible strategy
for AQs accumulation. M. citrifolia cell suspension cultures were
treated with azetidine-2-carboxylic acid (A2C; 25 and 50_M) and
thiazolidine-4-carboxylic acid (T4C; 100 and 200_M), two proline
analogs. All treatments except from A2C 25_M showed a higher
AQs content (P < 0.05) compared to the control line, while only
T4C 200_M treatment increased total phenolics (TP) content after
a six-day culture. After ten days of culture, both T4C treatments
enhanced AQs and TP production compared to control. Accumulation
of proline was significantly increased by all treatments after ten days
of culture (P < 0.01). These findings could be correlated with PPP stimulation.
doi:10.1016/j.nbt.2009.06.807
doi:10.1016/j.nbt.2009.06.807