GIULIETTI Ana Maria
congresos y reuniones científicas
A culture system design to study shear stress effects on Rubia tinctorum cell suspension cultures
BUSTO, V.; CALABRÓ LOPEZ, A; GIULIETTI, A M; RODRIGUEZ TALOU, J; MERCHUK, J.C
San Miguel de Tucuman
Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Shear stress is used as a mechanical elicitor to induce secondary metabolism in plants. We previously found that shear stress elicits anthraquinones (AQs) production in Rubia tinctorum suspension cultures growing in a stirred tank bioreactor. To study mechanical elicitation mechanisms a large number of experimental units working in parallel are necessary. The aim was to design a down-scaled and simple culture system which allows replacing bioreactor experiments with shaked flasks. To induce a similar intensity of shear stress to the one reached into the bioreactor, 100 mL baffled shake flasks on an orbital shaker at 360 rpm were used. Controls were carried out in 100 mL baffled flasks agitated at 100 rpm. Cultures were grown at 25 °C in presence or absence of light. Biomass concentration, cell death and AQs production were evaluated. A decrease in biomass concentration (28%) compared to control cultures was observed. Cell death increased in 60% and in 40% after 7 days of shear stress in presence and absence of light respectively. AQs accumulation increased up to 155% and 216% compared with control after 14 days of shear stress in presence and absence of light respectively. These results show that baffled flasks system elicit AQs production in a similar way as bioreactors in R. tinctorum suspension cultures and could be useful in the study of mechanical elicitation mechanisms.