CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
DESIGN OF A CULTURE SYSTEM TO STUDY ANTHRAQUINONES ELICITATION UNDER SHEAR STRESS IN RUBIA TINCTORUM CELL SUSPENSION CULTURES
Autor/es:
BUSTO, V. D; CALABRÓ LÓPEZ, A.; RODRÍGUEZ TALOU, J.; GIULIETTI, A. M.; MERCHUK J.C.
Lugar:
Rosario
Reunión:
Congreso; REDBIO 2009; 2009
Resumen:
Elicitation of plant cell cultures is an effective strategy to enhance the production of many pharmaceutical compounds, secondary metabolites among them. Some abiotic elicitors have been used to induce secondary metabolite accumulation in many plant species, which include shear stress. Rubia tinctorum suspension cultures produce anthraquinones (AQs), secondary metabolites, which are important in the pharmaceutical and food industries. We have found previously that shear stress elicited anthraquinones production in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm(1). The study of the mechanisms involved in shear stress elicitation would require a more complex experimental design. For this purpose, the bioreactors available become a limiting step. The aimof this work was to design a down-scaled and simple culture systemwhich allows replacing bioreactor experiments with shaked flasks to study shear stress elicitation inR. tinctorum cell suspension cultures.Rubia tinctorum suspension cultures produce anthraquinones (AQs), secondary metabolites, which are important in the pharmaceutical and food industries. We have found previously that shear stress elicited anthraquinones production in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm(1). The study of the mechanisms involved in shear stress elicitation would require a more complex experimental design. For this purpose, the bioreactors available become a limiting step. The aimof this work was to design a down-scaled and simple culture systemwhich allows replacing bioreactor experiments with shaked flasks to study shear stress elicitation inR. tinctorum cell suspension cultures.R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm(1). The study of the mechanisms involved in shear stress elicitation would require a more complex experimental design. For this purpose, the bioreactors available become a limiting step. The aimof this work was to design a down-scaled and simple culture systemwhich allows replacing bioreactor experiments with shaked flasks to study shear stress elicitation inR. tinctorum cell suspension cultures.(1). The study of the mechanisms involved in shear stress elicitation would require a more complex experimental design. For this purpose, the bioreactors available become a limiting step. The aimof this work was to design a down-scaled and simple culture systemwhich allows replacing bioreactor experiments with shaked flasks to study shear stress elicitation inR. tinctorum cell suspension cultures.R. tinctorum cell suspension cultures.