GIULIETTI Ana Maria
congresos y reuniones científicas
Estimation of biosurfactant production by Pseudomonas sp. growing on three different substrates
FRANCO LIPORACE ; DEBORA CONDE MOLINA; SUSANA VÁZQUEZ; GIULIETTI, ANA MARÍA; CARLA QUEVEDO
Mar del Plata
Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015
The microorganism used in this study was isolated from a chronically hydrocarbon-contaminated area in Campana (Bs.As.). Experiments were carried out in Erlenmeyer flasks andwere divided in two stages: initially, the strain was cultured for 48h in a minimal saline medium(MSM) supplemented with glucose (Glu; 20g/l) or glycerol (Gly; 3%v/v) as sole carbon andenergy source. Next, biomass produced with each substrate was transferred to a fresh MSMsupplemented with a mixture of three commercial hydrocarbons (HC), and the process wasmonitored for 72h. Biomass and biosurfactant production throughout the experiment wereestimated by cell dry weight and direct measurement of surface tension (ST) methods. Resultsshowed that bacteria was able to grow using all the carbon sources tested, reachingconcentrations of 1,29g/l and 0,92g/l in Glu and Gly respectively, and 0,24g/l and 0,32g/l in HCrespectively. Regarding ST measurement, culture supernatant showed a marked decrease in STvalue when the bacteria grew on Glu and Gly (46,30% and 54,72%). No decline in ST value wasobserved when HC was used. Results suggest that the isolated microorganism might be used toperform a biosurfactant production using renewable carbon sources (like Gly), with the aim ofdesigning a bioremediation strategy of hydrocarbon contaminated sites with native strains.