CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
Isolation of microorganisms for biosurfactant production
Autor/es:
LIPORACE FRANCO; QUEVEDO CV; GIULIETTI A M; OLIVERA JUAN
Reunión:
Simposio; ECB 16; 2014
Resumen:
Microorganisms with surfactant producing ability were isolated from hydrocarbon-contaminated soil and water in order to carry out processes for biosurfactant production.The samples were obtained from a petroleum distillery (RHASA) located at Campana, (Argentina). Isolation was performed by enrichment cultures in a mineral salt medium (MSM) containing 4,5% of a mixture of three different hydrocarbons (HC) or polluted lagoon water (AgLag) as both carbon and energy source. The strains isolated were cultured in 250 ml erlenmeyer flasks containing 25 ml of MSM supplemented with HC (pH 7.00) and incubated at 25 ◦C±2 ◦Cin orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 ◦C±2 ◦Cin orbital shaker at 150 rpm for 7 days. The biosurfactant producing ability of isolated microorganisms was estimated by emulsification capacity, surface tension measurement using Du Nouy ring tensiometer, oil spreading techniques, drop collapse method and haemolytic activity. Only six of the isolated strains were able to reduce the growth media surface tension in more than 40%. One of them, Ag.A.1HC strain, was cultured in a stirred tank bioreactor (NewBrunswick BioFlo115) containing 2500 ml MSM medium with a mixture of HC, operating at 200 rpm and 25 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 ◦C±2 ◦C. Biomass concentration and surface tension were evaluated during the process. A decrease in the surface tension value of the cellfree supernatant was observed. The initial and final values were 46,85mN/m and 34,90mN/m, respectively. The highest cell concentration was found between day 5 and 7 of culture. According to these results the Ag.A.1 HC strain, isolated from hydrocarboncontaminated water would have a potential use in both production of biosurfactant and bioremediation processes of environments contaminated with hydrocarbons. http://dx.doi.org/10.1016/j.nbt.2014.05.1910 PF-23