CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
Brugmansia candida hairy root cultures in bioreactors as an
Autor/es:
CARDILLO, A.; OTALVARO A.M.; BUSTO, V.; RODRIGUEZ TALOU, J; VELAZQUEZ, M.; GIULIETTI A.M.
Reunión:
Simposio; Brugmansia candida hairy root cultures in bioreactors as an; 2010
Resumen:
Brugmansia candida is a South American native plant that produces tropane alkaloids, such as hyoscyamine, anisodamine (6-hydroxyhyoscyamine) and scopolamine. These alkaloids were traditionally used in medicine because of their anticholinergic activity. Recently there were described further therapeutical properties for anisodamine renewing the interest in these alkaloids. Nowadays, these alkaloids are produced by the exploitation of producer plants due to the cost and complexity that chemical synthesis produces tropane alkaloids, such as hyoscyamine, anisodamine (6-hydroxyhyoscyamine) and scopolamine. These alkaloids were traditionally used in medicine because of their anticholinergic activity. Recently there were described further therapeutical properties for anisodamine renewing the interest in these alkaloids. Nowadays, these alkaloids are produced by the exploitation of producer plants due to the cost and complexity that chemical synthesis is a South American native plant that produces tropane alkaloids, such as hyoscyamine, anisodamine (6-hydroxyhyoscyamine) and scopolamine. These alkaloids were traditionally used in medicine because of their anticholinergic activity. Recently there were described further therapeutical properties for anisodamine renewing the interest in these alkaloids. Nowadays, these alkaloids are produced by the exploitation of producer plants due to the cost and complexity that chemical synthesis S486 Special Abstracts / Journal of Biotechnology 150S (2010) S1?S576Special Abstracts / Journal of Biotechnology 150S (2010) S1?S576 represents. The scaling up of hairy root cultures is an interesting strategy for the pharmaceutical production of these compounds instead of the isolation from plants what could be affected by external factors such as temperature or pathogens. The aim of this work was to study the anisodamine and scopolamine production by B. candida hairy roots immobilized in a plastic mesh submerged into a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. candida hairy roots immobilized in a plastic mesh submerged into a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. B. candida hairy roots immobilized in a plastic mesh submerged into a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. hairy roots immobilized in a plastic mesh submerged into a 1.5 l stirred tank as an alternative production system. A volume of 1.2 L of Gamborg B5/2 culture medium was inoculated with 10 g FW of 20-days-old hairy roots. The culture was aerated by a stainless steel sparger located below the turbine by an airflow rate of 0.5 vvm. The alkaloids were analyzed and quantified by HPLC with UV detection. It was found that bioreactor cultures produced an increased biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. The maximum concentration of anisodamine was 10.05±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system. ±0.76 mg/gDWbeing the predominant alkaloid at the harvest day (20 d). The results obtained are promissory for the development of an anisodamine production system.