GIULIETTI Ana Maria
congresos y reuniones científicas
Phytofermentation processes in stirred tank bioreactors by Rubia tinctorum suspension
BUSTO, V; CALABRÓ LÓPEZ, A.; RODRÍGUEZ TALOU J; GIULIETTI A M; MERCHUK J.C.
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Considering the complexity in the chemical synthesis of some secondary products, plant cell cultures represent an alternative for industrial application. However, an impediment of the practice of large-scale culture in stirred tanks is the shear stress. Rubia tinctorum suspension cultures produce anthraquinones (AQs) which are important in the pharmaceutical and food industries. The aim of this work was to study the performance of R. tinctorum suspension cultures in a stirred tank bioreactor including the evaluation of shear stress effects. A 1.5 L stirred tank bioreactor agitated at 150, 450 and 800 rpm was used. Controls were carried out in 100 mL shake flasks agitated at 100 rpm. Cell growth and AQs production were evaluated. Specific growth rate (µ) attained in shake flasks cultures was 27% and 42% higher than those obtained in the bioreactor cultures at 450 and 150 rpm, respectively. Cells cultured at 800 rpm were not able to grow into the bioreactor. Cell cultures were mechanically elicitated by shear stress at 450 and 800 rpm increasing AQs biosynthesis by 230% and 243%, respectively. AQs volumetric productivity (Qp) at 450 was 167% higher than that achieved in shake flasks. R. tinctorum cells showed moderately sensitivity in phytofermentative processes. The metabolic capabilities showed by these plant cells could be used as a potential strategy for industrial AQs production.