CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
Phytofermentation processes in stirred tank bioreactors by Rubia tinctorum suspension
Autor/es:
BUSTO, V; CALABRÓ LÓPEZ, A.; RODRÍGUEZ TALOU J; GIULIETTI A M; MERCHUK J.C.
Reunión:
Congreso; XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2011
Resumen:
Considering  the  complexity  in  the  chemical  synthesis  of  some  secondary  products,  plant cell cultures represent an alternative for industrial application. However, an impediment of the  practice  of  large-scale  culture  in  stirred  tanks  is  the  shear  stress.  Rubia  tinctorum suspension  cultures  produce  anthraquinones  (AQs)  which  are  important  in  the pharmaceutical and food industries. The aim of this work was to study the performance of R.  tinctorum  suspension  cultures  in  a  stirred  tank  bioreactor  including  the  evaluation  of shear  stress  effects.  A  1.5  L  stirred  tank  bioreactor  agitated  at  150,  450  and  800  rpm  was used.  Controls  were  carried  out  in  100  mL  shake  flasks  agitated  at  100  rpm.  Cell growth and  AQs  production  were  evaluated.  Specific  growth  rate  (µ)  attained  in  shake  flasks cultures was 27% and 42% higher than those obtained in the bioreactor cultures at 450 and 150 rpm, respectively. Cells cultured at 800 rpm were not able to grow into the bioreactor. Cell  cultures  were  mechanically  elicitated  by  shear  stress  at  450  and  800  rpm  increasing AQs  biosynthesis  by  230%  and  243%,  respectively.  AQs  volumetric  productivity  (Qp)  at 450  was  167%  higher  than  that  achieved  in  shake  flasks.  R.  tinctorum  cells  showed moderately  sensitivity  in  phytofermentative  processes.  The metabolic capabilities showed by these plant cells could be used as a potential strategy for industrial AQs production.