CONTRATADOS
GIULIETTI Ana Maria
congresos y reuniones científicas
Título:
Transient expression of dengue virus envelope protein in a plant system
Autor/es:
MARTÍNEZ, C; GIULIETTI, A M; RODRÍGUEZ TALOU J.; MASSON H
Reunión:
Simposio; 14th International Biotechnology Symposium and Exhibition; 2010
Resumen:
The utilization of plants as bioreactors presents several advantages related with its simplicity, biosafety and low cost. It retains the advantages of eukaryotic expression systems, as post-translational modifications, and prokaryotic expression systems, as scalability and economical production. However, long time-scale and low yield of recombinant proteins have been the main bottlenecks in plant-made biopharmaceuticals. A developed technique known as Magnifection can offset these disadvantages. This transient expression technology is based on replication of viral vectors delivered to the plant by Agrobacterium and allows very fast production, high recombinant protein expression levels. Dengue virus (DV) serotype 2 envelope glycoprotein (E) is the main protein associated with viral entry and the induction of immunity. In order to produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, E protein was expressed using this technologyAgrobacterium and allows very fast production, high recombinant protein expression levels. Dengue virus (DV) serotype 2 envelope glycoprotein (E) is the main protein associated with viral entry and the induction of immunity. In order to produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, E protein was expressed using this technology