CONTRATADOS
GIULIETTI Ana Maria
capítulos de libros
Título:
Biosynthesis of Sesquiterpene Lactones in Plants and Metabolic Engineering for Their Biotechnological Production
Autor/es:
MARIA PERASSOLO; ALEJANDRA CARDILLO; VICTOR BUSTO ; ANA MARIA GIULIETTI; JULIÁN RODRÍGUEZ TALOU
Libro:
Sesquiterpene Lactones
Editorial:
Springer International
Referencias:
Año: 2018; p. 47 - 91
Resumen:
Abstract In the present chapter, we review some aspects of the biosynthesis ofsesquiterpene lactones and its regulation in different medicinal and aromatic plantsused in the pharmaceutical industry. In this sense, we describe the mevalonate andthe 2-C-methyl-D-erythritol 4-phosphate pathways, which generate the correspondingisoprenoid precursors (isopentenyl diphosphate and dimethylallyl diphosphate),as well as the late pathways that lead to sesquiterpene lactone biosynthesis. Thischapter also analyses the role of the transcription factors involved in the regulationof sesquiterpene lactone biosynthesis and the different biotechnological approachesthat have been developed for sesquiterpene lactone production. In vitro plant cellcultures (comprising micropropagation and plant cell suspension, shoot and rootcultures) have emerged as a production platform for many plant secondary metabolites,since they allow their production under controlled conditions and shorter productioncycles. The characterisation and isolation of genes involved in the regulationof sesquiterpene lactone biosynthetic pathways have allowed the design of metabolicengineering strategies to increase the production of these metabolites.Moreover, we discuss different strategies to increase sesquiterpene lactone productionthrough genetic engineering. We also focus on the metabolic engineering of theartemisinin biosynthetic pathway in Artemisia annua. This metabolic pathway hasbecome a model system not only for the biotechnological production of sesquiterpenelactones but also for the improvement of other plant secondary metabolicpathways.Finally, we analyse the successful expression of the complete artemisinin biosynthetic pathway in Escherichia coli and Saccharomyces cerevisiae, which hasled to the efficient accumulation of artemisinic acid in these microorganisms