CONTRATADOS
GIULIETTI Ana Maria
artículos
Título:
Comparison of the influence of different elicitors on hyoscyamine and scopolamine content in hairy root cultures Brugmansia candida
Autor/es:
PITTA-ALVAREZ, S.; MARCONI P; GIULIETTI A M
Revista:
In Vitro Cell. Dev. Biol. -Plant
Editorial:
SPRINGER
Referencias:
Año: 2003 vol. 39 p. 640 - 640
Resumen:
Summary  Hairy roots of Brugmansia candida were exposed to different elicitors, such as pectinase, B. candida root homogenate, Hormonema ssp. homogenate, and the acetate control buffer. Pectinase increased intracellular hyoscyamine (200?300%) and the release of both alkaloids up to 1500% (scopolamine) and 1100% (hyoscyamine). However, the increment observed in both alkaloids in roots with the acetate control buffer was superior than with pectinase, obtaining increases of 700% in hyoscyamine and 200% in scopolamine. The B. candida root homogenate enhanced the accumulation (50?600%) and specific production of both alkaloids (ca. 150%). Hormonema ssp. homogenates induced different responses according to the original medium in which the fungus was cultured. The effect of each elicitor is discussed. Key words  scopolamine - hyoseyamine - biotie elicitors - tropane alkaloids -  Brugmansia candida