CONTRATADOS
GIULIETTI Ana Maria
artículos
Título:
Enhancement of anthraquinone production inMorinda citrifoliacell suspension cultures after stimulation of the proline cycle with two proline analogs
Autor/es:
QUEVEDO CV; PERASSOLO M; GIULIETTI AM; RODRÍGUEZ TALOU J
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 34 p. 571 - 571
ISSN:
0141-5492
Resumen:
Abstract Synthesis of anthraquinones (AQs) involves the shikimate and 2-C-methyl-D-erythritol 4-phosphate pathways. The proline cycle is linked to the pentose phosphate pathway (PPP) to generate NADPH needed in the first steps of this pathway. The effect of two proline analogs, azetidine-2-carboxylic acid (A2C) and thiazolidine-4-carboxylic acid (T4C), were evaluated in Morinda citrifoliasuspension cultures. Both analogs gave higher proline accumula-tion after 6 and 10 days (68 and 179% after 6 days with A2C at 25 and 50lM, respectively, and 111% with T4C added at 100lM). Induction of the proline cycle increased the AQ content after 6 days (*40% for 50lM A2C and 100lM T4C). Whereas A2C (50 lM) increased only AQ production, T4C also enhanced total phenolics. However, no induction of the PPP was observed with any of the treatments. This pathway therefore does not limit the supply of carbon skeletons to secondary metabolic pathways