Scopolamine, anisodamine and hyoscyamine production

CARDILLO, A.B.; OTALVARO A.M.; BUSTO, V; RODRÍGUEZ TALOU J.; VELÁSQUEZ LOZANO,M E; GIULIETTI A M

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Año: 2010 vol. 45 p. 1577 - 1577

1359-5113

Hyoscyamine, anisodamine (6â-hydroxyhyoscyamine) and scopolamine are tropane alkaloids(6â-hydroxyhyoscyamine) and scopolamine are tropane alkaloids  produced by plants belonging to the Solanaceae family such as Brugmansia candida. Theseproduced by plants belonging to the Solanaceae family such as Brugmansia candida. These  alkaloids were traditionally used in medicine because of their anticholinergic activity. Furtheralkaloids were traditionally used in medicine because of their anticholinergic activity. Further  therapeutical properties for anisodamine were recently described renewing the interest in thesetherapeutical properties for anisodamine were recently described renewing the interest in these alkaloids. The scaling up of hairy root cultures is an interesting strategy for the pharmaceuticalalkaloids. The scaling up of hairy root cultures is an interesting strategy for the pharmaceutical  production of these compounds instead of the isolation from plants. In this work, B. candida hairyproduction of these compounds instead of the isolation from plants. In this work, B. candida hairy  roots were cultured in a modified 1.5 L stirred tank in order to analyze an alternative productionroots were cultured in a modified 1.5 L stirred tank in order to analyze an alternative production system of scopolamine and anisodamine. It was found that these cultures produced an increasedsystem of scopolamine and anisodamine. It was found that these cultures produced an increased  biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks.biomass and alkaloids concentration compared to the processes carried out in Erlenmeyer flasks. Anisodamine was the predominant alkaloid reaching a maximum concentration of 10.05 ± 0.76Anisodamine was the predominant alkaloid reaching a maximum concentration of 10.05 ± 0.76  mg/g DW in modified bioreactor culture system. The results obtained in this work are potentiallymg/g DW in modified bioreactor culture system. The results obtained in this work are potentially applicable for the rational scale-up of the processapplicable for the rational scale-up of the process