CONTRATADOS
GIULIETTI Ana Maria
artículos
Título:
Increasing anthraquinone production by overexpression of 1-deoxy-D-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia
Autor/es:
QUEVEDO, C; PERASSOLO, M; ALECHINE, E; CORACH, D.; GIULIETTI A.M.; RODRÍGUEZ TALOU J.
Revista:
BIOTECHNOLOGY LETTERS
Editorial:
SPRINGER
Referencias:
Año: 2010 vol. 32 p. 997 - 997
ISSN:
0141-5492
Resumen:
 A Morinda citrifolia cell line was obtained by overexpresion of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs).This cell line increasedAQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. by overexpresion of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs).This cell line increasedAQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs).This cell line increasedAQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. enzyme of the metabolic pathway of anthraquinones (AQs).This cell line increasedAQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. effects of the overexpression of the dxs gene also resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. resulted in increased levels of dxsmRNAtranscripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls. DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonialyase activity were evaluated and were significantly higher in the transgenic line than in controls.