GIULIETTI Ana Maria
congresos y reuniones científicas
Análisis de rutas metabólicas secundarias en suspensiones celulares de Rubia tinctorum
JULIANA PARSONS; ANA MARIA GIULIETTI; JULIAN RODRIGUEZ TALOU
Congreso; XL Congreso de SAIB; 2004
High intracellular proline levels deregulate proline synthesis in plants which leads to an overexpressed pentose phosphate pathway which results in an increasing carbon flux through the shikimate pathway. The stimulation of shikimate pathway produced an increase in phenylpropanoid related secondary metabolites (phenolics) in plants. Chorismate, which is the end product of the shikimate pathway, becomes the branch point for the synthesis of phenylpropanoid and anthraquinones (AQs) in Rubia tinctorum secondary metabolism. We tested the effect of proline addition in plant suspension cultures of R. tinctorum in order to study the competition between the two above mentioned secondary metabolic pathways. Suspension cultures were treated with proline at different concentrations 5 and 25 mM. High levels of proline ( 5 and 25 mM) showed a significant decrease on AQs accumulation ( 35 % an 50 %) and an increase in phenolics concentration. The addition of the phenylalanine ammonia liase inhibitor, 2-aminoindan-2-phosphonic acid, at 40 and 100 mM resulted in an increase of AQs concentration in suspension cultures of 18 and 40 % respectively. These results show that is possible to increase AQs production at expense of the phenylpropanoid pathway in R. tinctorum suspension cultures.