capítulos de libros
In vitro hairy root cultures as a tool for phytoremediation research
Phytoremediation: Methods and Reviews
Human Press
Lugar: New Jersey; Año: 2007; p. 161 - 173
Plant model systems are needed to conduct basic laboratory studies, prior to field applications of phytoremediation. In vitro plant cultures are a useful tool for research purposes. This chapter focuses on the generation of hairy root cultures and their use as a laboratory root model for the in vitro study of the removal of aromatic compounds. A protocol for the generation of hairy root cultures of Armoracia lapathifolia, L. by infection with Agrobacterium rhizogenes (a soil born bacterial strain that induces the differentiation of roots at the infection spots) is described. A second protocoI describes the application of hairy root cultures in assays for studying the removal of organic compounds, exemplified with phenol, a model organic contaminant. A. lapathifolia roots contain a high level of peroxidases (E.C., enzymes which are known to be involved in the detoxification of phenols and other aromatic compounds. Briefly, the cultures are exposed to different concentrations of the contaminant under study and the remaining amounts of it and some physiological parameters are monitored along defined time intervals. This experimental procedure permits to estimate the capability of a plant species to remove a contaminant and also the main variables that may affect the remediation process. This information is essential for assessing the feasibility of a remediation process prior to its field application. This can be done at relatively low analytical expenses and in short periods of time with the use of in vitro plant models. Hence, the hairy root culture constitutes a valuable tool for phytoremediation research and development.