Comparative effect of galactooligosaccharides (GOS) from two different diets matrix in bone heath of growing rats

MARIANA SEIJO; GABRIEL BRYK; MAGALI ZENI CORONEL M; MARINA SOLEDAD BONANNO; CLAUDIA INÉS VÉNICA; MARÍA LUZ PITA MARTIN DE PORTELA; BERGAMINI C.; VERÓNICA IRMA WOLF; M. CRISTINA PEROTTI

Jornada; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

In previous studies we demonstrated the effectiveness of GOS to increase calcium (Ca) absorption and bioavailability, and to improve bone retention. Objective: to compare the effect of galactooligosaccharides (GOS) from two different food matrix on bone formation and quality during normal growth in rats. Methods: Male weaning rats were divided in 3 groups and fed until 40 days of age: AIN-93G control diet (C), GOS mixed with fructooligosaccharides (FOS) in a proportion of GOS/FOS® 9:1 (PM) or GOS mixed with probiotic bacteria’s containing in an experimental yogurt diet (EY). Femur length (LF) (cm) and its Ca and phosphate (Pi) content; bone mineral density (BMD) and (BMC) of the total skeleton (tSk), lumbar spine (lSp) and proximal tibia (pT) by densitometry; bone volume percentage (BV%) and epiphyseal (GPC. Th) and hypertrophic (HpZ.Th) cartilages (μm) by histology; maximum fracture strength (N), and bone stiffness (N/mm) by biomechanical method. Shapiro-Wilk and Levene normality tests were applied, and ANOVA was performed to determine differences between groups using SPSS 19.0. p