CHANGES IN TRYPANOSOMA CRUZI PHOSPHOLIPID TURNOVER ARE INDUCED BY PARASITE CONTACT TO CELL MEMBRANES.

MN GARRIDO,; G RACAGNI,; BMI PEREIRA,; DANIEL LUJAN, HUGO; MA RODRIGUEZ,; HAUEN DE BRONIA, DELIA; EE MACHADO,

PARASITOLOGY RESEARCH

Año: 2000 vol. 86 p. 96 - 100

0932-0113

To investigate the possibility that cell contact could initiate a series of signals in both the host cell and the flagellate protozoan Trypanosoma cruzi, we studied [32P]-phospholipid turnover during parasite interaction with cellular membranes in vitro. Lipid alterations were produced in the parasite during the initial period of contact with the plasma membranes of human erythrocytes. In the presence of calcium an increment in phosphatidylethanolamine was observed with a concomitant decrease in phosphatidic acid fractions, whereas these modifications were not observed in the absence of calcium. There was an evident decrease in phosphatidylcholine and a shift in the phosphatidylinositol/lysophosphatidylethanolamine fraction among the phospholipids of major turnover in the absence or presence of calcium. Among the minor labeled species, lysophosphatidylcholine reached levels that duplicated control values, whereas the amounts of lysophosphatidylinositol, phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate diminished by over 50%. All of these variations indicate that the parasite´s contact with plasma membranes induces changes involving T. cruzi phospholipids and suggest the participation of these compounds in the activation of intracellular mechanisms that might be important during the life cycle of this parasite.