INVESTIGADORES
PEREZ Claudio Javier
artículos
Título:
Development of pH-responsive biopolymer-silica composites loaded with Larrea divaricata Cav. extract with antioxidant activity
Autor/es:
M.I.ALVAREZ ECHAZU; C.E. OLIVETTI; I.PERALTA; M.R.ALONSO; C. ANESINI; C. J. PÉREZ; G.S. ALVAREZ; M.F. DESIMONE
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 169 p. 82 - 82
ISSN:
0927-7765
Resumen:
A detailed study of biomaterials is mandatory to comprehend their feasible biomedical applications interms of drug delivery and tissue regeneration. Particularly, mucoadhesive biopolymers such as chitosan(chi) and carboxymethylcellulose (CMC) have become interesting biomaterials regards to their biocom-patibility and non-toxicity for oral mucosal drug delivery. In this work, pH-responsive biopolymer-silicacomposites (Chi-SiO2, Chi-CMC-SiO2) were developed. These two types of composites presented a dif-ferent swelling behavior due to the environmental pH. Moreover, the nanocomposites were loaded withaqueous Larrea divaricata Cav. extract (Ld), a South American plant which presents antioxidant prop-erties suitable for the treatment of gingivoperiodontal diseases. Chi-CMC-SiO2composites showed thehighest incorporation and reached the 100% of extract release in almost 4 days while they preserved theirantioxidant properties. In this study, thermal and swelling behavior were pointed out to show the distinctwater-composite interaction and therefore to evaluate their mucoadhesivity. Furthermore, a cytotoxicitytest with 3T3 fibroblasts was assessed, showing that in both composites the addition of Larrea divaricataCav. extract increased fibroblast proliferation. Lastly, preliminary in vitro studies were performed withsimulated body fluids. Indeed, SEM-EDS analysis indicated that only chi-SiO2composite may providean environment for possible biomineralization while the addition of CMC to the composites discour-aged calcium accumulation. In conclusion, the development of bioactive composites could promote theregeneration of periodontal tissue damaged throughout periodontal disease and the presence of silicananoparticles could provide an environment for biomineralization