BOSIO Valeria Elizabeth
congresos y reuniones científicas
Carrageenan based microspheres for oral controlled release of Enrofloxacin in biomedical applications
M.CACICEDO; G.A. ISLAN; V. E. BOSIO; A. CAUERHFF; G.R. CASTRO
Pcia de Taiwan, Taipei
Congreso; 5th International Conference on Industrial Bioprocesses 2012; 2012
Enrofloxacin (ENRO) is a third generation fluoroquinolone which has a broad spectrum of anti-microbial activity mainly used in veterinary medicine. Current oral administration of ENRO is commonly associated to gastric and intestinal problems, in addition to its toxicity at high concentrations. In this work a biopolymeric matrix based on natural and non toxic biopolymers was developed in order to improve the ENRO release profile for oral administration and reduce the undesirable drug side effects. For this purpose, several biopolimeric matrices were assayed, finding that the more stable interaction with ENRO was obtained with carrageenan (70 % at 1 hour). Carrageenan-ENRO interaction at different pHs were studied, finding that at pH 4.0 a degree of interaction close to 70% was reached. Based on that, carrageenan was mixed with other biopolymers, pectin and alginate, to make blended microspheres by ionic gelation. Although, these systems have an encapsulation capacity of the drug around 40 %, the release profile reached the 100 % at two hours in simulated intestinal media. Several optimization assays were preformed: HM pectin and oleic acid coating showed a decrease of 20 % in the amount of ENRO release. Additionally, hybrid microparticles of Calcium Carbonate and carrageenan (at 0.5 and 1 % wt/vol in reaction media) were synthesized. Microspheres of 5 micrometers diameter (Optical Microscopy) loaded with ENRO, showed a sustainable release profile in simulated intestinal fluids, reaching a 65 and 59 % of ENRO released at 3 hours for carrageenan at 0.5 and 1 % wt/vol respectively. The improvement in the ENRO release kinetics suggests the system as possible candidate for biomedical applications, minimizing the toxic effects of antibiotic treatment.