SEREMETA Katia Pamela
congresos y reuniones científicas
Nanoencapsulación de antirretrovirales en nanopartículas de poli(epsilon-caprolactona) para la optimización de la farmacoterapia del virus de la inmunodeficiencia humana
Mar del Plata
Congreso; XVIII Congreso Argentino de Bioingeniería, VII Jornadas de Ingeniería Clínica, 2° Taller de Órganos Artificiales, Biomateriales e Ingeniería de Tejidos; 2011
Institución organizadora:
Regional Mar del Plata de la Sociedad Argentina de Bioingeniería y Facultad de Ingeniería de la Universidad Nacional de Mar del Plata
According to the latest World Health Organization fact sheets, approximately 40 million people are chronically infected with the HIV worldwide. A successful antiretroviral therapy is associated with a dramatic decrease in the Acquired Immunodeficiency Syndrome (AIDS)-associated mortality. Didanosine (ddI), a synthetic purine nucleoside analogue that inhibits the reverse transcriptase, is used in combination with other antiretroviral drugs as a part of the highly active antiretroviral therapy (HAART). DdI quickly undergoes hydrolysis in the stomach to hypoxanthine, an inactive form. Thus, its oral bioavailability is relatively low (20-40%). This work will present preliminary results on the encapsulation of the drug within poly (є-caprolactone) nanoparticles as an approach to improve its chemical stability and oral bioavailability. Several techniques (e.g., water-in oil-in-oil emulsion/solvent diffusion/evaporation) were assessed to produce the drug-loaded nanoparticles. Sizes were between 200-250 nm as determined by Dynamic Light Scattering. The total ddI payload was measured by HPLC and the morphology analyzed by SEM. Due to its high water solubility the encapsulation of the drug was not easily accomplished. In this context, the most important limitations in the encapsulation process will be also discussed.