Interaction between the Cell Cycle and a Cell Fate Decision System in Yeast Saccharomyces cerevisiae: a Systems Biology Approach

ALICIA GRANDE; EDGAR ALTSZYLER; ARIEL CHERNOMORETZ; ALEJANDRO COLMAN LERNER

Los Cocos

Congreso; The Second South American Symposium on Signal Transduction and Translational Medicine; 2012

SISTAM

In haploid yeast, mating Cdc28/Cln2 activity is essential to pass the START G1 physically supported by a network of interacting approximation, we used a set of nonlinear ordinary pheromone triggers a fate checkpoint, while Cdc28/Cln3 is required earlier in G1 to proteins, evolving in space and time according to differential equations (ODE) that describe the dynamics decision: arrest of the cell cycle in G1 and initiation of drive the expression of Cln2. This has two important fundamental laws of reaction, diffusion and transport, of the simultaneously occurring reactions. We tested our mating events. To do that, the MAP kinase of the consequences: a) inhibition of Cdc28/Clns causes cells to here we propose a basic mathematical model, with a model using fluorescent microscopy, measuring pheromone response pathway (PRP) Fus3 activates the arrest in G1, and b) since Cdc28/Cln2 can block small number of parameters and dynamical variables, fluorescent proteins used as reporters of the PRP and cyclin dependent kinase inhibitor Far1, which binds to and inhibits all three G1 cyclins complexes, Cdc28/Clns. pheromone response, its inhibition by Far1 acts as a positive feedback loop. Because the signaling pathway is that reproduces the observed biological phenomena: the morphology for the cell cycle. interaction between the cell cycle and the PRP. As a first