INVESTIGADORES
COLMAN LERNER Alejandro Ariel
artículos
Título:
Scaffold number in yeast signaling system sets tradeoff between system output and dynamic range
Autor/es:
TY THOMSON; KIRSTEN BENJAMIN; ALAN BUSH; TONYA LOVE; DAVID PINCUS; ORNA RESNEKOV; RICHARD YU; ANDREW GORDON; ALEJANDRO COLMAN LERNER; DREW ENDY; ROGER BRENT
Revista:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Editorial:
NATL ACAD SCIENCES
Referencias:
Año: 2011
ISSN:
0027-8424
Resumen:
While the proteins that comprise many cell signaling systems are now known, less is known about quantitative protein levels, and existing measurements often vary by a factor of 10 or more. Here we devised improved quantification methods to measure protein abundances in the Saccharomyces cerevisiae pheromone response pathway, an archetypical cell signaling system. With these methods, we were able to limit variation between independent measurements of protein abundance to a factor of two. Using these numbers, we constructed quantitative models to identify and investigate behaviors of the pheromone response system that were sensitive to precise protein abundances. We found that the difference between the maximum and basal signaling output (dynamic range) of the pheromone response mitogen-activated protein (MAP) kinase cascade is strongly sensitive to the abundance of Ste5, the MAP kinase scaffold protein, and that absolute system output depends on the amount of Fus3, the MAP kinase. Additional analysis and experiment suggests that scaffold abundance sets a tradeoff between maximum system output and system dynamic range