COLMAN LERNER Alejandro Ariel
Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates.
ALEJANDRO COLMAN-LERNER (CORRESPONDING AUTHOR); TINA CHIN; ROGER BRENT
Año: 2001 vol. 107 p. 739 - 750
In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs. Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription factor to the daughter nucleus. Ectopic localization of active Ace2 to mother nuclei is sufficient to activate daughter-specific genes in mothers. Eight genes are daughter-specific under the tested conditions, while two are daughter-specific only in saturated cultures. Some daughter-specific gene products contribute to cell separation by degrading the cell wall. These experiments define programs of gene expression specific to daughters and describe how those programs are controlled.