Transcriptome analyses of Solanum pimpinellifolium LA0722 at three ripening stages

CACCHIARELLI, P.; ELIZABETH, TAPIA; GUILLERMO RAUL PRATTA

ROma

Congreso; SOL International Online Meeting 2020.; 2020

SOL Genomics

n a previous report, transcriptome of S. pimpinellifolium LA0722 was aligned as S. lycopersicum cv. Heinz 1706 as a reference. In this report, we have realigned LA0722 transcriptome using as a reference the S, pimpinellifolium LA4791 genome, recently available to the scientific community. The objective was to compare results of both transcriptome analyses, one made on a same species reference genome and the other, on a related species genome. In both cases, the green mature transcriptome was used to normalize the data. Differences in the number of over-expressed genes and under-expressed genes were detected when comparing both analyses, the smallest values corresponding to alignment of LA0722 transcriptome against the same species LA4701 genome (7024 and 6676 differentially expressed genes in LA0722 between breaker and green mature stages and between red ripe and green matures stages, respectively, vs. 7313 and 6929 in the same conditions when using cv. Heinz 1706 as the reference genome). Our results pointed out the importance of using an appropriate reference for alignments of new transcriptomes.