INVESTIGADORES
PRATTA Guillermo Raul
artículos
Título:
Molecular diversity in selected banana clones (Musa AAA ?Cavendish?): adapted to the subtropical environment of Formosa Province (Argentina)
Autor/es:
ERMINI, J.L.; TENAGLIA, G.; GUILLERMO RAUL PRATTA
Revista:
American Journal of Plant Sciences
Editorial:
SCIRP
Referencias:
Año: 2018 vol. 9 p. 2504 - 2513
ISSN:
2158-2750
Resumen:
Banana production dates from the 1920s in Argentina. Rhizomes were introduced and dispersed by immigrants from bordering countries in the Northern provinces. There is scarce information on its genetic diversity to assist in crop breeding programs; hence studies of genetic structure between populations and individuals are fundamental for future use. Molecular markers assess the diversity of the crop. This study employed Amplified Fragment Length Polymorphism to investigate the genetic variation in local banana plants from farmer?s fields. Forty-four rhizomes (selected from a total of 860 plants for being stable for production) were used as plant material and 6 primer combinations selected in a previous report were used. Polymorphic fragments present in a given genotype were assigned 1 and those absent were assigned 0. The matrix generated was analyzed by univariate and multivariate analysis. A total 540 bands were scored, of which 100% were polymorphic. The number of the pattern duplicated bands was 23 and twenty-one amplicons were exclusive to banana plants collected in the same farmer?s field. Hierarchical clustering and principal coordinates analyses showed differences between genotypes. The cophenetic correlation of the cluster was 0.63 while the 18% of the total molecular variation was explained by the two principalcoordinates. These analyses evidence genetic diversity of the crop accordingto farmer?s field. In agreement to this observation, analysis of molecular variance revealed that 8.9% of the variation was found among farmer populations and 91.1% within farmer populations. In conclusion, introduction of banana into the subtropical zone was associated with a broader genetic variation in order to increase the genetic homeostasis necessary for adapting the crop to the suboptimal environment of Northeastern Argentinean Formosa Province.