Advances in Biotechnology: Tomato as a Plant Model System

ZORZOLI, ROXANA; PRATTA, G.R.; RODRÍGUEZ, G. R.; PICARDI, L. A.

Functional Plant Science and Biotechnology

Global Science Books

Lugar: Tokyo; Año: 2007 vol. 1 p. 146 - 159

1749-0472

This review reports some aspects of advances in biotechnology such as tissue culture, genetic engineering, molecular markers, and the new approach of the “omics” in tomato. In vitro regeneration has been a subject of research because of the commercial value of the crop and its amenability for further improvement via genetic manipulation. Numerous studies on plant regeneration from a wide range of tissues of wild and cultivated tomato germplasm have been conducted. Several genes involved in fruit ripening and others traits have been characterized and genetically engineered plants were successful obtained. One of the main uses of molecular markers has been the construction of linkage maps. Linkage maps have been utilized to identify chromosomal regions that contained genes controlling simple trait and QTL. DNA markers that are tightly linked to important genes are used as molecular tools for marker-assisted-selection (MAS) in tomato breeding. The latest trend is to combine QTL mapping with methods in functional genomics. There are wide collections of ESTs. The DNA microarrays analysis is also used to study the expression of many genes. Nowadays the proteomics and metabolomics allow identifying biochemical factors underlying important traits for tomato breeding programs.In vitro regeneration has been a subject of research because of the commercial value of the crop and its amenability for further improvement via genetic manipulation. Numerous studies on plant regeneration from a wide range of tissues of wild and cultivated tomato germplasm have been conducted. Several genes involved in fruit ripening and others traits have been characterized and genetically engineered plants were successful obtained. One of the main uses of molecular markers has been the construction of linkage maps. Linkage maps have been utilized to identify chromosomal regions that contained genes controlling simple trait and QTL. DNA markers that are tightly linked to important genes are used as molecular tools for marker-assisted-selection (MAS) in tomato breeding. The latest trend is to combine QTL mapping with methods in functional genomics. There are wide collections of ESTs. The DNA microarrays analysis is also used to study the expression of many genes. Nowadays the proteomics and metabolomics allow identifying biochemical factors underlying important traits for tomato breeding programs.