Induction of ammonium metabolizing enzymes correlates with fruit shelf life in tomato crosses among cultivated, mutant and exotic germplasms

PRATTA, GUILLERMO; ZORZOLI, ROXANA; PICARDI, LILIANA AMELIA; VALLE, ESTELA MARTA

International Journal of Plant Breeding

Global Science Books

Lugar: Tokyo; Año: 2007 vol. 1 p. 70 - 73

1752-3478

In a previous report, glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were differentially induced in fruits at two ripening stages among tomato lines with different shelf life (SL). The objective of this work was to analyze SL and the induction of GS and GDH in the pericarp tissue at the mature-green and the ripe stages in different tomato genotypes, including a set of hybrids and their parents. The L. esculentum parents were the standard ripening cultivar Caimanta, which produces round fresh-marketable fruits, and two inbred lines, N and R, which are recessive for the nor and rin mutations respectively. The exotic parent Ceras belonged to the wild L. esculentum var. cerasiforme. Four crosses were assayed: F1 (Caimanta x N), F1 (Caimanta x Ceras), F1 (N x Ceras), and F1 (N x R). The concomitant presence of GS and GDH was found in the pericarp of mature-green and ripe tomato fruits from N, R, Ceras, F1 (Caimanta x Ceras), F1 (N x Ceras), and F1 (N x R). All these genotypes had long SL. Particularly, in ripe fruits of N and R, the genotypes with the longest SL, two isoforms of GDH were detected. This unique pattern was not observed in any of the other genotypes. The F1 (Caimanta x N) had a quite shorter SL and presented a differential induction pattern: while GS was found just in mature-green fruits, GDH was present at both ripening stages. In Caimanta, the genotype with shortest SL, GS was present only at the mature-green stage and GDH at the ripe stage. Differences for the induction pattern  of GS and GDH found in tomato fruits at two ripening stages could be correlated with their SL values.