INVESTIGADORES
PRATTA Guillermo Raul
artículos
Título:
Genotype variations of early protein expression during tomato in vitro culture
Autor/es:
MARCHIONNI BASTÉ, EZEQUIEL; RODRÍGUEZ, G. R.; PRATTA, G.R.; ZORZOLI, R
Revista:
International Journal of Plant Developmental Biology
Editorial:
Global Science Books
Referencias:
Lugar: Tokio; Año: 2009 vol. 3 p. 10 - 14
ISSN:
1749-4753
Resumen:
Polymorphism in total protein patterns is an indicator of variability for genes associated with regeneration ability. The objective was to characterize the early expression of proteins involved in tomato organogenesis from leaflet explants of divergent taxonomic genotypes with different regeneration abilities. Solanum lycopersicum cv. ‘Caimanta’ as the highest regenerating genotype, accession LA722 of S. pimpinellifolium as the lowest regenerating genotype and F1 were assayed. In vitro culture was performed according to the standard protocol for tomato. Twenty samples of each genotype were analyzed at different days of incubation (from 1 to 10 days). Uncultured explants were the experimental tester. Total proteins were extracted from these samples in phosphate buffer and then separated by SDS-PAGE. Polymorphism was found for polypeptides of 76.6, 55.5, 52.1, 49.7, 44.5, 27.9, 24.7, 24.1, and 19.5 kDa, which accounted for 56% of the total protein patterns. Polymorphic polypeptides between incubation periods were those of 76.6, 49.7, 44.5, and 24.7 kDa. Polymorphic polypeptides between genotypes were those of 52.1, 27.9, 24.1, and 19.5 kDa. Polymorphism of total protein patterns during the first days of incubation, and between divergent taxonomic genotypes with different regeneration ability, indicated variability of genetic expression of the in vitro response.