MODULATION OF THE IMMUNE RESPONSE BY A NOVEL AMNIOTIC MEMBRANE DERIVATIVE IN A HUMAN MONOCYTIC CELL LINE

XIMENA GUERBI; ELIZAGARAY MAIA; LAURA CARBALLO; GRISELDA MORENO; FLAVIA MICHELINI; ALEJANDRO BERRA

Mar del Plata

Congreso; Reunión de Sociedades Biomedicas: Sociedad Argentina de Investigación Clínica (SAIC); Sociedad Argentina de Inmunología (SAI); Sociedad Argentina de Fisiología (SAFIS); 2022

Sociedad Argentina de Investigación Clínica (SAIC); Sociedad Argentina de Inmunología (SAI); Sociedad Argentina de Fisiología (SAFIS)

Macrophages can acquire distinct functionalities, depending on the type of activating stimuli they are exposed to. They might be activated in a proinflammatory way (M1), or might undertake an alternative activation pathway in order to promote tissue remodeling and repair (M2). Amniotic Membrane has been used as a biological dressing. We found that the healing time of complex skin wounds has been surprisingly shortened in patients treated with a novel lyophilized and sterilized amniotic membrane patch. To understand the mechanisms involved, we characterized the cytokines profile secreted by THP-1 monocytes differentiated to macrophage during 72 h treatment with 50 ng/ml PMA followed by 100 ng/ml LPS activation, with or without the addition of rehydrated patches supernatants to the culture wells. After 6 and 20 h, IL-6, IL-8, TNF-𝛂 and IL-10 were quantified by ELISA. Although no significant differences were observed in IL-10 secretion after 6 h of treatment, we noticed a significant increase after the long treatment. Both IL-6 and TNF-𝛂 decreased in this condition, as well. Eventhough, IL-8 was upmodulated beyond the level induced by LPS in both conditions. Cell viability was verified by MTT assay, showing not just the lack of cytotoxicity but also an increased viability in the amniotic membrane products treated wells. In all cases, ANOVA followed by Tuckey test, p2). To sum up, these derivatives seem to induce a shift in the activation profile of macrophages, from M1 to M2, which might be involved in a faster and better wound healing process.