Immune response triggered by a novel vaccine against pertussis.

GRISELDA MORENO; MARIA EMILA GAILLARD; MARTINEZ SABATER DAVID; DANIELA BOTTERO; AGUSTINA ERREA; BARTEL ERIKA; BRAVO SOL; CARRIQUIRIBORDE FRANCISCO; CELINA CASTUMA; MARTIN RUMBO; DANIELA HOZBOR

Buenos Aires

Congreso; . IV LASID Meeting. LXIII Argentinean Immunology Society Meeting. II French-Argentinean Immunology Meeting; 2015

Pertussis remains a public health problem despite the availability of classical whole-cell vaccines (wP) and acellular vaccines (aP). Increased pertussis cases were detected even in countries with high vaccination coverage. This increment was attributed to waning immunity and pathogen adaptation. It was estimated that the average duration of vaccine protection using a calendar with only aP doses is ∼3 years. Individuals vaccinated with wP or wP plus aP boosters showed a longer duration of immunity. This could be due to the different immune responses induced by these two types of vaccines. While wP promotes Th1/Th17 responses, aP induces strong Th2 response with low Th1/Th17 contribution.Recently we designed a new vaccine based on outer membrane vesicles (OMVs) derived from Bordetella pertussis, which behaved in animal model as an excellent vaccine candidate. Here, we evaluated both humoral and cell-mediated immune responses induced by OMVs-vaccine. Significant amounts of anti-Bp antibodies were detected in OMVs-vaccine immunized mice with low ratio of IgG1/IgG2a. Regarding cellular response, we detected IFN-ɤ in the supernatant of OMVs-stimulated spleen cells from both OMVs (1,000±200 pg/ml) and wP-immunized mice (5,000±850 pg/ml). Weak response was observed in aP-immunized mice (50±15 pg/ml). In contrast, IL-5 secretion was mainly detected in spleen cells from aP mice (1,500±250 pg/ml). High secretion of IL-17 was observed in OMVs-vaccine and wP immunized mice (1,500±300 pg/ml and 2,500±450 pg/ml, respectively) compared to aP mice (200±50 pg/ml). In conclusion, OMVs-vaccine elicits a Th1/Th2 and Th17 immune response profile while also inducing a robust antibody response.