Activation of PPAR a and PPAR B/D regulates Sertoli cell metabolism

REGUEIRA MARIANA; RIERA M FERNANDA; GALARDO M NOEL; PELLIZZARI ELIANA H; CIGORRAGA SELVA B; MERONI SILVINA B

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2014 vol. 382 p. 271 - 281

0303-7207

The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR B/D activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742-pharmacological activators of PPAR a and PPAR B/D respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-CoA dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR B/D activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR a activation participates in the regulation of FA metabolism. On the other hand, PPAR B/D activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.